Recombinant human RPL31 protein

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL31 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

60S ribosomal protein L31 isoform 1, L31

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

16.9 kDa (148aa) confirmed by MALDI-TOF

Additionnal Information

RPL31, 60S ribosomal protein L31 isoform 1, L31, ATGP2433-10 µg, ATGP2433-20 µg, ATGP2433-50 µg, ATGP2433-100 µg, ATGP2433-250 µg, ATGP2433-500 µg, ATGP2433-1 mg, ATGP2433-10, ATGP2433-20, ATGP2433-50, ATGP2433-100, ATGP2433-250, ATGP2433-500, ATGP2433-1

References & Citations

Ewing RM, Chu P, et al. (2007) . Mol Syst Biol. 3:89.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000984

Uniprot Accession Number

P62899

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPAKKG GEKKKGRSAI NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLVT YVPVTTFKNL QTVNVDEN