Recombinant human ACAD8 protein
ACAD8 is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. Recombinant human ACAD8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.1 kDa (416aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hendrickson, S.L., et al. (2010) PLoS ONE 5 (9), E12862; ; Battaile, K.P., et al. (2004) J. Biol. Chem. 279 (16), 16526-16534;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_055199
Uniprot Accession Number
Q9UKU7
Species
Human
AA Sequence
Frequently Asked Questions
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