Recombinant human PMF1-BGLAP protein
This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
PMF1-BGLAP protein isoform 2, PMF1-BGLAP readthrough
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
26.2 kDa (234aa)
Additionnal Information
References & Citations
Udeshi ND. et al. (2012) Mol Cell Proteomics. 11:148-159.; ; Strausberg RL. et al. (2002) Proc Natl Acad Sci u S A. 99:16899-16903;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001186591
Uniprot Accession Number
U3KQ54
Species
Human
AA Sequence
Frequently Asked Questions
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