Recombinant human PMF1-BGLAP protein

This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

PMF1-BGLAP protein isoform 2, PMF1-BGLAP readthrough

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

26.2 kDa (234aa)

Additionnal Information

PMF1-BGLAP, PMF1-BGLAP protein isoform 2, PMF1-BGLAP readthrough, ATGP2417-10 µg, ATGP2417-20 µg, ATGP2417-50 µg, ATGP2417-100 µg, ATGP2417-250 µg, ATGP2417-500 µg, ATGP2417-1 mg, ATGP2417-10, ATGP2417-20, ATGP2417-50, ATGP2417-100, ATGP2417-250, ATGP2417-500, ATGP2417-1

References & Citations

Udeshi ND. et al. (2012) Mol Cell Proteomics. 11:148-159.; ; Strausberg RL. et al. (2002) Proc Natl Acad Sci u S A. 99:16899-16903;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001186591

Uniprot Accession Number

U3KQ54

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEASSA NLGSGCEEKR HEGSSSESVP PGTTISRVKL LDTMVDTFLQ KLVAAGSYQR FTDCYKCFYQ LQPAMTQQIY DKFIAQLQTS IREEISDIKE EGNLEAVLNA LDKIVEEGKV RKEPAWRPSG IPEKDLHSVM APYFLQQRDT LRRHVQKQEA ENQQLADAVL AGRRQVEELQ LQVQAQQQAW QVRSPAVQSP AKVQPLCPSR RAAR