Recombinant human UGT8 protein

UDP glycosyltransferase 8, also known as uGT8, catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Recombinant human uGT8 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

UDP glycosyltransferase 8, CGT, uGT4, UDP-galactose ceramide galactosyltransferase, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, Ceramide UDP-galactosyltransferase, Cerebroside synthase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

61.6 kDa (544aa)

Additionnal Information

UGT8, uDP glycosyltransferase 8, CGT, uGT4, ATGP2414-10 µg, ATGP2414-20 µg, ATGP2414-50 µg, ATGP2414-100 µg, ATGP2414-250 µg, ATGP2414-500 µg, ATGP2414-1 mg, ATGP2414-10, ATGP2414-20, ATGP2414-50, ATGP2414-100, ATGP2414-250, ATGP2414-500, ATGP2414-1

References & Citations

Bosio A., et al. (1996) Genomics. 34:69-75; ; Kapitonov D.E., et al. (1997) Biochem. Biophys. Res. Commun. 232:449-453;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_003351

Uniprot Accession Number

Q16880

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSAKIIIVP PIMFESHMYI FKTLASALHE RGHHTVFLLS EGRDIAPSNH YSLQRYPGIF NSTTSDAFLQ SKMRNIFSGR LTAIELFDIL DHYTKNCDLM VGNHALIQGL KKEKFDLLLV DPNDMCGFVI AHLLGVKYAV FSTGLWYPAE VGAPAPLAYV PEFNSLLTDR MNLLQRMKNT GVYLISRLGV SFLVLPKYER IMQKYNLLPE KSMYDLVHGS SLWMLCTDVA LEFPRPTLPN VVYVGGILTK PASPLPEDLQ RWVNGANEHG FVLVSFGAGV KYLSEDIANK LAGALGRLPQ KVIWRFSGPK PKNLGNNTKL IEWLPQNDLL GHSKIKAFLS HGGLNSIFET MYHGVPVVGI PLFGDHYDTM TRVQAKGMGI LLEWKTVTEK ELYEALVKVI NNPSYRQRAQ KLSEIHKDQP GHPVNRTIYW IDYIIRHNGA HHLRAAVHQI SFCQYFLLDI AFVLLLGAAL LYFLLSWVTK FIYRKIKSLW SRNKHSTVNG HYHNGILNGK YKRNGHIKHE KKVK