Recombinant human FHL3 protein

FHL3 is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human FHL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Four and a half LIM domains 3, Four and a half LIM domains 3, SLIM2, NP_004459, SLIM-2, Skeletal muscle LIM-protein 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.6 kDa (303aa) confirmed by MALDI-TOF

Additionnal Information

FHL3, Four and a half LIM domains 3, Four and a half LIM domains 3, SLIM2, NP_004459, SLIM-2, Skeletal muscle LIM-protein 2, ATGP2411-10 µg, ATGP2411-20 µg, ATGP2411-50 µg, ATGP2411-100 µg, ATGP2411-250 µg, ATGP2411-500 µg, ATGP2411-1 mg, ATGP2411-10, ATGP2411-20, ATGP2411-50, ATGP2411-100, ATGP2411-250, ATGP2411-500, ATGP2411-1

References & Citations

Morgan M.J., et al. (1999) Biochem. Biophys. Res. Commun. 255:245-250; ; Morgan M.J., et al. (1996) Biochem. Biophys. Res. Commun. 225:632-638;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004459

Uniprot Accession Number

Q13643

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSESFDC AKCNESLYGR KYIQTDSGPY CVPCYDNTFA NTCAECQQLI GHDSRELFYE DRHFHEGCFR CCRCQRSLAD EPFTCQDSEL LCNDCYCSAF SSQCSACGET VMPGSRKLEY GGQTWHEHCF LCSGCEQPLG SRSFVPDKGA HYCVPCYENK FAPRCARCSK TLTQGGVTYR DQPWHRECLV CTGCQTPLAG QQFTSRDEDP YCVACFGELF APKCSSCKRP IVGLGGGKYV SFEDRHWHHN CFSCARCSTS LVGQGFVPDG DQVLCQGCSQ AGP