Recombinant human DNALI1 protein

DNALI1 is the human homolog of the Chlamydomonas inner dynein arm gene, p28. The precise function of this gene is not known, however, it is a potential candidate for immotile cilia yndrome (ICS) . ultrastructural defects of the inner dynein arms are seen in patients with ICS. Immotile mutant strains of Chlamydomonas, a biflagellated algae, exhibit similar defects. Recombinant human DNALI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Axonemal dynein light intermediate polypeptide 1, dJ423B22.5, hp28, P28

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.2 kDa (303aa) confirmed by MALDI-TOF

Additionnal Information

DNALI1, axonemal dynein light intermediate polypeptide 1, dJ423B22.5, hp28, P28, ATGP2410-10 µg, ATGP2410-20 µg, ATGP2410-50 µg, ATGP2410-100 µg, ATGP2410-250 µg, ATGP2410-500 µg, ATGP2410-1 mg, ATGP2410-10, ATGP2410-20, ATGP2410-50, ATGP2410-100, ATGP2410-250, ATGP2410-500, ATGP2410-1

References & Citations

Kastury K., et al (1997) . Biochem. J. Clin. Endocrinol. Metab. 82:3047-3053; ; Sjoeblom T., et al (2006) . Science 314:268-274 (2006) ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003453

Uniprot Accession Number

O14645

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMVTANKA HTGQGSCWVA TLASAMIPPA DSLLKYDTPV LVSRNTEKRS PKARLLKVSP QQPGPSGSAP QPPKTKLPST PCVPDPTKQA EEILNAILPP REWVEDTQLW IQQVSSTPST RMDVVHLQEQ LDLKLQQRQA RETGICPVRR ELYSQCFDEL IREVTINCAE RGLLLLRVRD EIRMTIAAYQ TLYESSVAFG MRKALQAEQG KSDMERKIAE LETEKRDLER QVNEQKAKCE ATEKRESERR QVEEKKHNEE IQFLKRTNQQ LKAQLEGIIA PKK