Recombinant mouse RAPSN protein

43 kDa receptor-associated protein of the synapse, also known as RAPSN, is expressed in the postsynaptic membrane of skeletal muscle. RAPSN is required for the clustering of nicotinic acetylcholine receptors (nAChR) . It self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs) . RAPSN interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain. This protein modifies trafficking of AChR within the cell. Overexpression inhibits agrin-induced AChR clustering pathway. Recombinant human RAPSN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

43 kDa receptor-associated protein of the synapse, 43kDa, Nraps, Raps, rapsyn

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

48.8 kDa (435aa)

Additionnal Information

RAPSN, 43 kDa receptor-associated protein of the synapse, 43kDa, Nraps, Raps, rapsyn, ATGP2405-10 µg, ATGP2405-20 µg, ATGP2405-50 µg, ATGP2405-100 µg, ATGP2405-250 µg, ATGP2405-500 µg, ATGP2405-1 mg, ATGP2405-10, ATGP2405-20, ATGP2405-50, ATGP2405-100, ATGP2405-250, ATGP2405-500, ATGP2405-1

References & Citations

Maimone M M., et al. (1999) Mol Cell Neurosci. 14:340-354.; ; Han H., et al. (1999) J Neurocytol. 28:763-775;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Neuroscience

NCBI Accession Number

NP_033049

Uniprot Accession Number

P12672

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGQDQTK QQIEKGLQLY QSNQTEKALQ VWMKVLEKGS DLVGRFRVLG CLVTAHSEMG RYKEMLKFAV VQIDTARGLE DADFLLESYL NLARSNEKLC EFHKTISYCK TCLGLPGTRA GAQLGGQVSL SMGNAFLGLS LFQKALESFE KALRYAHNND DTMLECRVCC SLGSFYAQVK DYEKALFFPC KAAELVNDYG KGWSLKYRAM SQYHMAVAYR LLGHLGSAME CCEESMKIAL QHGDRPLQAL CLLCFADIHR SRGDLETAFP RYDSAMSIMT EIGNRLGQVH VLLGVAKCWM ARKVQDKALD AIEKAQDLAE EVGNKLSQLK LHCLSESIYR SKGLQRELRT HVVRFHECVE ETELYCGLCG ESIGERNSRL QALPCSHIFH LRCLQNNGTR SCPNCRRSSM KPGFV