Recombinant human Fc epsilon RI alpha/FCER1A protein

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. FCER1A represents the alpha subunit. Recombinant human FCER1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, FcepsilonRI alpha chain, FcERI, IgE Fc receptor subunit alpha, FCER1A, FCE1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

23.4 kDa (203aa) confirmed by MALDI-TOF

Additionnal Information

Fc epsilon RI a, high affinity immunoglobulin epsilon receptor subunit alpha precursor, high affinity immunoglobulin epsilon receptor subunit alpha precursor, FCE1A, FcERI, ATGP2394-10 µg, ATGP2394-20 µg, ATGP2394-50 µg, ATGP2394-100 µg, ATGP2394-250 µg, ATGP2394-500 µg, ATGP2394-1 mg, ATGP2394-10, ATGP2394-20, ATGP2394-50, ATGP2394-100, ATGP2394-250, ATGP2394-500, ATGP2394-1

References & Citations

ZHANG, Y., et al. (2012) Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 47 (4), 289-293; ; Zhou, J., et al. (2012) Hum. Immunol. 73 (3), 301-305;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_001992.1

Uniprot Accession Number

P12319

Species

Human

AA Sequence

VPQKPKV SLNPPWNRIF KGENVTLTCN GNNFFEVSST KWFHNGSLSE ETNSSLNIVN AKFEDSGEYK CQHQQVNESE PVYLEVFSDW LLLQASAEVV MEGQPLFLRC HGWRNWDVYK VIYYKDGEAL KYWYENHNIS ITNATVEDSG TYYCTGKVWQ LDYESEPLNI TVIKAPREKY WLQ