Recombinant human SYT5 protein

Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules. Recombinant human SYT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Synaptotagmin-5, Synaptotagmin V

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.6 kDa (301aa) confirmed by MALDI-TOF

Additionnal Information

SYT5, Synaptotagmin-5, Synaptotagmin V, ATGP2390-10 µg, ATGP2390-20 µg, ATGP2390-50 µg, ATGP2390-100 µg, ATGP2390-250 µg, ATGP2390-500 µg, ATGP2390-1 mg, ATGP2390-10, ATGP2390-20, ATGP2390-50, ATGP2390-100, ATGP2390-250, ATGP2390-500, ATGP2390-1

References & Citations

Craxton M.A., Olsen A., et al. (1997), Genomics 42:165-169

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl, 2mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_003171

Uniprot Accession Number

O00445

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSLGRLQYS LDYDFQSGQL LVGILQAMGL AALDLGGSSD PYVRVYLLPD KRRRYETKVH RQTLNPHFGE TFAFKVPYVE LGGRVLVMAV YDFDRFSRND AIGEVRVPMS SVDLGRPVQA WRELQAAPRE EQEKLGDICF SLRYVPTAGK LTVIVLEAKN LKKMDVGGLS DPYVKVHLLQ GGKKVRKKKT TIKKNTLNPY YNEAFSFEVP CDQVQKVQVE LTVLDYDKLG KNEAIGRVAV GAAAGGAGLR HWADMLANPR RPIAQWHSLR PPDRVRLLPA P