Recombinant human GAGE12F protein
GAGE12F belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells. Recombinant human GAGE12F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
G antigen 12F, OTTHuMG00000024147
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by BCA assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.4 kDa (140aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gjerstorff M.F., et al (2008) . Tissue Antigens 71:187-192; ; The MGC Project Team (2004) Genome Res. 14:2121-2127;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_001091875
Uniprot Accession Number
O76087
Species
Human
AA Sequence
MSWRGRS TYYWPRPRRY VQPPEMIGPM RPEQFSDEVE PATPEEGEPA TQRQDPAAAQ EGEDEGASAG QGPKPEAHSQ EQGHPQTGCE CEDGPDGQEM DPPNPEEVKT PEEGEKQSQC
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