Recombinant human GAGE12F protein
GAGE12F belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells. Recombinant human GAGE12F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
G antigen 12F, OTTHuMG00000024147
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by BCA assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.4 kDa (140aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gjerstorff M.F., et al (2008) . Tissue Antigens 71:187-192; ; The MGC Project Team (2004) Genome Res. 14:2121-2127;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_001091875
Uniprot Accession Number
O76087
Species
Human
AA Sequence
MSWRGRS TYYWPRPRRY VQPPEMIGPM RPEQFSDEVE PATPEEGEPA TQRQDPAAAQ EGEDEGASAG QGPKPEAHSQ EQGHPQTGCE CEDGPDGQEM DPPNPEEVKT PEEGEKQSQC
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
AKAP12 (A-kinase Anchor Protein 12, AKAP-12, A-kinase Anchor Protein 250kD, AKAP 250, Gravin, Myasthenia Gravis Autoantigen, AKAP250, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621) (MaxLight 650)
Human Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) Protein
Human Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) Protein
Human Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) Protein