Recombinant human PLSCR3 protein

PLSCR3 may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. This protein may play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. It seems to play a role in apoptosis, through translocation of cardiolipin from the inner to the outer mitochondrial membrane which promotes BID recruitment and enhances tBid-induced mitochondrial damages. Recombinant human PLSCR3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Phospholipid scramblase 3, PLS3, Ca (2+) -dependent phospholipid scramblase 3, PL scramblase 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.9 kDa (288aa)

Additionnal Information

PLSCR3, Phospholipid scramblase 3, PLS3, Ca (2+) -dependent phospholipid scramblase 3, PL scramblase 3, ATGP2384-10 µg, ATGP2384-20 µg, ATGP2384-50 µg, ATGP2384-100 µg, ATGP2384-250 µg, ATGP2384-500 µg, ATGP2384-1 mg, ATGP2384-10, ATGP2384-20, ATGP2384-50, ATGP2384-100, ATGP2384-250, ATGP2384-500, ATGP2384-1

References & Citations

He Y., et al (2007), J. Cell. Biochem. 101:1210-1221; ; Shibata H., et al 2008) J. Biol. Chem. 283:9623-9632;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_001188505

Uniprot Accession Number

Q9NRY6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGYLPP KGYAPSPPPP YPVTPGYPEP ALHPGPGQAP VPAQVPAPAP GFALFPSPGP VALGSAAPFL PLPGVPSGLE FLVQIDQILI HQKAERVETF LGWETCNRYE LRSGAGQPLG QAAEESNCCA RLCCGARRPL RVRLADPGDR EVLRLLRPLH CGCSCCPCGL QEMEVQAPPG TTIGHVLQTW HPFLPKFSIQ DADRQTVLRV VGPCWTCGCG TDTNFEVKTR DESRSVGRIS KQWGGLVREA LTDADDFGLQ FPLDLDVR