Recombinant human GNB3 protein

GNB3 is important regulator of alpha subunit, as well as of certain signal transduction receptor and effector. A single-nucleotide polymorphism (C825T) in this protein is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described. Recombinant human GNB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Guanine nucleotide-binding protein subunit beta-3, Guanine nucleotide binding protein (G protein), beta polypeptide 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

39.6 kDa (363aa)

Additionnal Information

GNB3, Guanine nucleotide-binding protein subunit beta-3, Guanine nucleotide binding protein (G protein), beta polypeptide 3, ATGP2382-10 µg, ATGP2382-20 µg, ATGP2382-50 µg, ATGP2382-100 µg, ATGP2382-250 µg, ATGP2382-500 µg, ATGP2382-1 mg, ATGP2382-10, ATGP2382-20, ATGP2382-50, ATGP2382-100, ATGP2382-250, ATGP2382-500, ATGP2382-1

References & Citations

Wedegaertner PB. et al. (1995) J. Biol. Chem. 270: 503-506.; ; Sartori M. et al. (2005) Ann. Ital. Med. Int. 19: 240-248.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_002066

Uniprot Accession Number

P16520

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AKLWDVREGT CRQTFTGHES DINAICFFPN GEAICTGSDD ASCRLFDLRA DQELICFSHE SIICGITSVA FSLSGRLLFA GYDDFNCNVW DSMKSERVGI LSGHDNRVSC LGVTADGMAV ATGSWDSFLK IWN