Recombinant human GNB3 protein
GNB3 is important regulator of alpha subunit, as well as of certain signal transduction receptor and effector. A single-nucleotide polymorphism (C825T) in this protein is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described. Recombinant human GNB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Guanine nucleotide-binding protein subunit beta-3, Guanine nucleotide binding protein (G protein), beta polypeptide 3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
39.6 kDa (363aa)
Additionnal Information
References & Citations
Wedegaertner PB. et al. (1995) J. Biol. Chem. 270: 503-506.; ; Sartori M. et al. (2005) Ann. Ital. Med. Int. 19: 240-248.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea
Scientific Category
Metabolism
NCBI Accession Number
NP_002066
Uniprot Accession Number
P16520
Species
Human
AA Sequence
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