Recombinant human CHCHD3 protein

Coiled-coil-helix-coiled-coil-helix domain containing protein 3, also known as CHCHD3, is required for maintenance of mitochondrial crista integrity and mitochondrial function. This protein may act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. It has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Recombinant human CHCHD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Coiled-coil-helix-coiled-coil-helix domain containing protein 3, MINOS3, PPP1R22

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

28.5 kDa (250aa) confirmed by MALDI-TOF

Additionnal Information

CHCHD3, Coiled-coil-helix-coiled-coil-helix domain containing protein 3, MINOS3, PPP1R22, ATGP2378-10 µg, ATGP2378-20 µg, ATGP2378-50 µg, ATGP2378-100 µg, ATGP2378-250 µg, ATGP2378-500 µg, ATGP2378-1 mg, ATGP2378-10, ATGP2378-20, ATGP2378-50, ATGP2378-100, ATGP2378-250, ATGP2378-500, ATGP2378-1

References & Citations

Liu H., et al. (2012) PLoS ONE. 7:E34832-E34832; ; Alkhaja A.K., et al. (2012) Mol. Biol. Cell. 23:247-257;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_060282

Uniprot Accession Number

Q9NX63

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGGTTST RRVTFEADEN ENITVVKGIR LSENVIDRMK ESSPSGSKSQ RYSGAYGASV SDEELKRRVA EELALEQAKK ESEDQKRLKQ AKELDRERAA ANEQLTRAIL RERICSEEER AKAKHLARQL EEKDRVLKKQ DAFYKEQLAR LEERSSEFYR VTTEQYQKAA EEVEAKFKRY ESHPVCADLQ AKILQCYREN THQTLKCSAL ATQYMHCVNH AKQSMLEKGG