Recombinant human UBC3B/UBE2R2 protein

UBE2R2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This protein is a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. Recombinant human UBE2R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2 R2, E2 ubiquitin-conjugating enzyme R2, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, CDC34B, UBC3B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.6 kDa (261aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CDC34B, E2 CDC34B, E2 ubiquitin conjugating enzyme R2, E2 ubiquitin-conjugating enzyme R2, E2CDC34B, E2-CDC34B, UBC3B, UBE2R2, Ubiquitin carrier protein R2, Ubiquitin conjugating enzyme E2 R2, Ubiquitin conjugating enzyme E2-CDC34B, Ubiquitin protein ligase R2, Ubiquitin-conjugating enzyme E2 R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin conjugating enzyme E2 CDC34B, Ubiquitin-conjugating enzyme E2 CDC34B, ATGP2374-10 µg, ATGP2374-20 µg, ATGP2374-50 µg, ATGP2374-100 µg, ATGP2374-250 µg, ATGP2374-500 µg, ATGP2374-1 mg, ATGP2374-010, ATGP2374-020, ATGP2374-050, ATGP2374-100, ATGP2374-250, ATGP2374-500, ATGP2374-01M

References & Citations

Yoshida, T., et al. (2010) Int. J. Mol. Med. 25 (4), 649-656; ; Oguri, M., et al. (2006) Am. J. Hypertens. 23 (1), 70-77;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_060281

Uniprot Accession Number

Q712K3

Species

Human

AA Sequence

MAQQQMT SSQKALMLEL KSLQEEPVEG FRITLVDESD LYNWEVAIFG PPNTLYEGGY FKAHIKFPID YPYSPPTFRF LTKMWHPNIY ENGDVCISIL HPPVDDPQSG ELPSERWNPT QNVRTILLSV ISLLNEPNTF SPANVDASVM FRKWRDSKGK DKEYAEIIRK QVSATKAEAE KDGVKVPTTL AEYCIKTKVP SNDNSSDLLY DDLYDDDIDD EDEEEEDADC YDDDDSGNEE S