Recombinant human HNRNPAB protein

HNRNPAB belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRNPAB, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Recombinant human HNRNPAB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heterogeneous nuclear ribonucleoprotein A/B isoform b, Heterogeneous nuclear ribonucleoprotein A/B, ABBP1; HNRPAB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33 kDa (308aa) confirmed by MALDI-TOF

Additionnal Information

HNRNPAB, Heterogeneous nuclear ribonucleoprotein A/B isoform b, Heterogeneous nuclear ribonucleoprotein A/B, ABBP1; HNRPAB, ATGP2371-10 µg, ATGP2371-20 µg, ATGP2371-50 µg, ATGP2371-100 µg, ATGP2371-250 µg, ATGP2371-500 µg, ATGP2371-1 mg, ATGP2371-10, ATGP2371-20, ATGP2371-50, ATGP2371-100, ATGP2371-250, ATGP2371-500, ATGP2371-1

References & Citations

Joeson L., Vikesaa J, et al. (2007), Mol. Cell. Proteomics 6:798-811

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004490

Uniprot Accession Number

Q99729

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSEAGEE QPMETTGATE NGHEAVPEGE SPAGAGTGAA AGAGGATAAP PSGNQNGAEG DQINASKNEE DAGKMFVGGL SWDTSKKDLK DYFTKFGEVV DCTIKMDPNT GRSRGFGFIL FKDAASVEKV LDQKEHRLDG RVIDPKKAMA MKKDPVKKIF VGGLNPEATE EKIREYFGEF GEIEAIELPM DPKLNKRRGF VFITFKEEEP VKKVLEKKFH TVSGSKCEIK VAQPKEVYQQ QQYGSGGRGN RNRGNRGSGG GGGGGGQGST NYGKSQRRGG HQNNYKPY