Recombinant E.coli ung protein

Ung, also known as uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. uracil bases occur from cytosine deamination or misincorporation of duMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E. coli ung protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Uracil-DNA-glycosylase, ECK2578, JW2564, UDG, UNG1, UNG2, HIGM4

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.1 kDa (252aa) confirmed by MALDI-TOF

Additionnal Information

Ung, uracil-DNA-glycosylase, ECK2578, JW2564, ATGP2365-10 µg, ATGP2365-20 µg, ATGP2365-50 µg, ATGP2365-100 µg, ATGP2365-250 µg, ATGP2365-500 µg, ATGP2365-1 mg, ATGP2365-10, ATGP2365-20, ATGP2365-50, ATGP2365-100, ATGP2365-250, ATGP2365-500, ATGP2365-1

References & Citations

Lindahl T. et al. (1977) J Biol Chem. 252 : 3286-3294.; ; Pearl, L. H. et al. (2000) Mutation research 460 : 165-181.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_417075

Uniprot Accession Number

P12295

Species

E.coli

AA Sequence

MANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE