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Recombinant human CAPZA2 protein

CAPZA2 is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end. Recombinant human CAPZA2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

F-actin-capping protein subunit alpha-2, CAPPA2, CAPZ

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.3 kDa (309aa)

Additionnal Information

CAPZA2, F-actin-capping protein subunit alpha-2, CAPPA2, CAPZ, ATGP2356-10 µg, ATGP2356-20 µg, ATGP2356-50 µg, ATGP2356-100 µg, ATGP2356-250 µg, ATGP2356-500 µg, ATGP2356-1 mg, ATGP2356-10, ATGP2356-20, ATGP2356-50, ATGP2356-100, ATGP2356-250, ATGP2356-500, ATGP2356-1

References & Citations

Eyers C.E., et al (2005) . Biochem. J. 389:127-135; ; Derivery E., et al (2009) . Dev. Cell 17:712-723

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M uREA

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_006127

Uniprot Accession Number

P47755

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMADLEEQ LSDEEKVRIA AKFIIHAPPG EFNEVFNDVR LLLNNDNLLR EGAAHAFAQY NLDQFTPVKI EGYEDQVLIT EHGDLGNGKF LDPKNRICFK FDHLRKEATD PRPCEVENAV ESWRTSVETA LRAYVKEHYP NGVCTVYGKK IDGQQTIIAC IESHQFQAKN FWNGRWRSEW KFTITPSTTQ VVGILKIQVH YYEDGNVQLV SHKDIQDSLT VSNEVQTAKE FIKIVEAAEN EYQTAISENY QTMSDTTFKA LRRQLPVTRT KIDWNKILSY KIGKEMQNA

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