Recombinant human SMCP protein

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. SMCP localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath. Recombinant human SMCP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sperm mitochondria-associated cysteine-rich protein, HSMCSGEN1, MCS, MCSP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

15.2 kDa (139aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

SMCP, Sperm mitochondria-associated cysteine-rich protein, HSMCSGEN1, MCS, MCSP, ATGP2354-10 µg, ATGP2354-20 µg, ATGP2354-50 µg, ATGP2354-100 µg, ATGP2354-250 µg, ATGP2354-500 µg, ATGP2354-1 mg, ATGP2354-10, ATGP2354-20, ATGP2354-50, ATGP2354-100, ATGP2354-250, ATGP2354-500, ATGP2354-1

References & Citations

Aho H., et al. (1996) Genomics. 32:184-190; ; Hawthorne S.K., et al. (2006) Genomics. 87:382-391

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM EDTA, 0.1mM PMSF

Scientific Category

Development

NCBI Accession Number

NP_109588

Uniprot Accession Number

P49901

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMCDQTKH SKCCPAKGNQ CCPPQQNQCC QSKGNQCCPP KQNQCCQPKG SQCCPPKHNH CCQPKPPCCI QARCCGLETK PEVSPLNMES EPNSPQTQDK GCQTQQQPHS PQNESRPSK