Recombinant human VPS4B protein
Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Recombinant human VPS4B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
51.8 kDa (468aa)
Additionnal Information
References & Citations
Scheuring S., et al. (2001) J Mol Biol. 312:469-480.; ; Perez O D., et al. (2001) Immunity. 15:687-690
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004860.2
Uniprot Accession Number
O75351
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
TSGA10IP sgRNA CRISPR Lentivector (Human) (Target 3)
