Recombinant human ORC6 protein

Origin recognition complex subunit 6, also known as ORC6, belongs to the origin recognition complex (ORC) . ORC is a highly conserved heterohexameric protein complex that associates with DNA at or near sites of initiation of DNA replication. All six ORC subunits are essential for initiation of DNA replication, and ORC may be involved in regulation of gene expression in response to stress. ORC6 is also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. Recombinant human ORC6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Origin recognition complex subunit 6, ORC6L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.5 kDa (275aa) confirmed by MALDI-TOF

Additionnal Information

ORC6, Origin recognition complex subunit 6, ORC6L, ATGP2351-10 µg, ATGP2351-20 µg, ATGP2351-50 µg, ATGP2351-100 µg, ATGP2351-250 µg, ATGP2351-500 µg, ATGP2351-1 mg, ATGP2351-10, ATGP2351-20, ATGP2351-50, ATGP2351-100, ATGP2351-250, ATGP2351-500, ATGP2351-1

References & Citations

Kreitz S., et al. (2000) J Biol Chem. 276:6337-6342; ; Mendez J., et al. (2000) Mol Cell Biol. 20:8602-8612.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_055136

Uniprot Accession Number

Q9Y5N6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGSELIG RLAPRLGLAE PDMLRKAEEY LRLSRVKCVG LSARTTETSS AVMCLDLAAS WMKCPLDRAY LIKLSGLNKE TYQSCLKSFE CLLGLNSNIG IRDLAVQFSC IEAVNMASKI LKSYESSLPQ TQQVDLDLSR PLFTSAALLS ACKILKLKVD KNKMVATSGV KKAIFDRLCK QLEKIGQQVD REPGDVATPP RKRKKIVVEA PAKEMEKVEE MPHKPQKDED LTQDYEEWKR KILENAASAQ KATAE