Recombinant human TAF10 protein

TAF10 is one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. Recombinant human TAF10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transcription initiation factor TFIID subunit 10, TAF2A; TAF2H, TAFII30

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

16.9 kDa (158aa)

Additionnal Information

TAF10, transcription initiation factor TFIID subunit 10, TAF2A; TAF2H, TAFII30, ATGP2346-10 µg, ATGP2346-20 µg, ATGP2346-50 µg, ATGP2346-100 µg, ATGP2346-250 µg, ATGP2346-500 µg, ATGP2346-1 mg, ATGP2346-10, ATGP2346-20, ATGP2346-50, ATGP2346-100, ATGP2346-250, ATGP2346-500, ATGP2346-1

References & Citations

Brand M., et al (1999) . J. Biol. Chem. 274:18285-18289; ; Gangloff Y.G., et al (2001) . Mol. Cell. Biol. 21:5109-5121;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006275

Uniprot Accession Number

Q12962

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT