Recombinant human TAF10 protein
TAF10 is one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. Recombinant human TAF10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Transcription initiation factor TFIID subunit 10, TAF2A; TAF2H, TAFII30
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
16.9 kDa (158aa)
Additionnal Information
References & Citations
Brand M., et al (1999) . J. Biol. Chem. 274:18285-18289; ; Gangloff Y.G., et al (2001) . Mol. Cell. Biol. 21:5109-5121;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006275
Uniprot Accession Number
Q12962
Species
Human
AA Sequence
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