Recombinant human TIPIN protein

TIPIN belongs to the CSM3 family. This protein is required for normal progression of S-phase and important for cell survival after DNA damage or replication stress. TIPIN may be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Recombinant human TIPIN, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

TIMELESS interacting protein, TIMELESS-interacting protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.9 kDa (324aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

TIPIN, TIMELESS interacting protein, TIMELESS-interacting protein, ATGP2342-10 µg, ATGP2342-20 µg, ATGP2342-50 µg, ATGP2342-100 µg, ATGP2342-250 µg, ATGP2342-500 µg, ATGP2342-1 mg, ATGP2342-10, ATGP2342-20, ATGP2342-50, ATGP2342-100, ATGP2342-250, ATGP2342-500, ATGP2342-1

References & Citations

Dheekollu, J., et al. (2011) J. Virol. 85 (12), 5853-5863; ; Kemp, M.G., et al. (2010) J. Biol. Chem. 285 (22), 16562-16571;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_060328

Uniprot Accession Number

Q9BVW5

Species

Human

AA Sequence

MLEPQEN GVIDLPDYEH VEDETFPPFP PPASPERQDG EGTEPDEESG NGAPVPVPPK RTVKRNIPKL DAQRLISERG LPALRHVFDK AKFKGKGHEA EDLKMLIRHM EHWAHRLFPK LQFEDFIDRV EYLGSKKEVQ TCLKRIRLDL PILHEDFVSN NDEVAENNEH DVTSTELDPF LTNLSESEMF ASELSRSLTE EQQQRIERNK QLALERRQAK LLSNSQTLGN DMLMNTPRAH TVEEVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR