Recombinant human ASB8 protein

Ankyrin repeat and SOCS box containing 8, also known as ASB8, may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recombinant human ASB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ankyrin repeat and SOCS box containing 8, PP14212

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.0 kDa (311aa) confirmed by MALDI-TOF

Additionnal Information

ASB8, Ankyrin repeat and SOCS box containing 8, PP14212, ATGP2341-10 µg, ATGP2341-20 µg, ATGP2341-50 µg, ATGP2341-100 µg, ATGP2341-250 µg, ATGP2341-500 µg, ATGP2341-1 mg, ATGP2341-10, ATGP2341-20, ATGP2341-50, ATGP2341-100, ATGP2341-250, ATGP2341-500, ATGP2341-1

References & Citations

Liu Y., et al. (2003) Biochem. Biophys. Res. Commun. 300:972-979; ; Ota T., et al. (2004) Nat. Genet. 36:40-45;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Ubiquitination

NCBI Accession Number

NP_077000

Uniprot Accession Number

Q9H765

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSSMWY IMQSIQSKYS LSERLIRTIA AIRSFPHDNV EDLIRGGADV NCTHGTLKPL HCACMVSDAD CVELLLEKGA EVNALDGYNR TALHYAAEKD EACVEVLLEY GANPNALDGN RDTPLHWAAF KNNAECVRAL LESGASVNAL DYNNDTPLSW AAMKGNLESV SILLDYGAEV RVINLIGQTP ISRLVALLVR GLGTEKEDSC FELLHRAVGH FELRKNGTMP REVARDPQLC EKLTVLCSAP GTLKTLARYA VRRSLGLQYL PDAVKGLPLP ASLKEYLLLL E