Recombinant human DNTTIP1 protein

DNTTIP1 binds DNA and enhances the activity of terminal deoxynucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template. Recombinant human DNTTIP1 fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Deoxynucleotidyltransferase terminal-interacting protein 1, C20orf167, dJ447F3.4, Tdif1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

39 kDa (352aa) confirmed by MALDI-TOF

Additionnal Information

DNTTIP1, Deoxynucleotidyltransferase terminal-interacting protein 1, C20orf167, dJ447F3.4, Tdif1, ATGP2338-10 µg, ATGP2338-20 µg, ATGP2338-50 µg, ATGP2338-100 µg, ATGP2338-250 µg, ATGP2338-500 µg, ATGP2338-1 mg, ATGP2338-10, ATGP2338-20, ATGP2338-50, ATGP2338-100, ATGP2338-250, ATGP2338-500, ATGP2338-1

References & Citations

Yamashita N, Shimazaki N, et al. (2001) . Genes Cells. 6 (7) :641-52.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_443183

Uniprot Accession Number

Q9H147

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGATGDA EQPRGPSGAE RGGLELGDAG AAGQLVLTNP WNIMIKHRQV QRRGRRSQMT TSFTDPAISM DLLRAVLQPS INEEIQTVFN KYMKFFQKAA LNVRDNVGEE VDAEQLIQEA CRSCLEQAKL LFSDGEKVIP RLTHELPGIK RGRQAEEECA HRGSPLPKKR KGRPPGHILS SDRAAAGMVW KPKSCEPIRR EGPKWDPARL NESTTFVLGS RANKALGMGG TRGRIYIKHP HLFKYAADPQ DKHWLAEQHH MRATGGKMAY LLIEEDIRDL AASDDYRGCL DLKLEELKSF VLPSWMVEKM RKYMETLRTE NEHRAVEAPP QT