Recombinant human AMPK gamma1/PRKAG1 protein
5'-AMP-activated protein kinase subunit gamma-1 isoform 1, also known as PRKAG1, is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Recombinant human PRKAG1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
5'-AMP-activated protein kinase subunit gamma-1 isoform 1, AMPKG, Protein kinase AMP-activated non-catalytic subunit gamma 1, AMPK subunit gamma-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
40 kDa (354aa)
Additionnal Information
References & Citations
Zaha VG., et al. (2012) Circ Res. 111 (6) :800-14; ; Za tara G., et al. (2008) Biochem Pharmacol. 76 (10) :1263-75.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002724
Uniprot Accession Number
P54619
Species
Human
AA Sequence
Frequently Asked Questions
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