Recombinant human NOB1 protein
In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5. 8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. Recombinant human NOB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
RNA-binding protein NOB1, RNA-binding protein NOB1, ART-4, MST158, MSTP158, NOB1P, PSMD8BP1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
49.1 kDa (435aa)
Additionnal Information
References & Citations
Zhang Y., et al. (2005) Kidney Int. 79:1138-1148; ; Daub H., et al. (2008) Mol. Cell. 31:438-448;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_054781
Uniprot Accession Number
Q9ULX3
Species
Human
AA Sequence
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