Recombinant human PP1 subunit R4/PPP1R3B protein

PPP1R3B is the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein. Recombinant human PPP1R3B protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Protein phosphatase 1 regulatory subunit 3B, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL, Protein phosphatase 1 regulatory subunit 4, PP1 subunit R4, Protein phosphatase 1 subunit GL, PTG, PPP1R4 GL, hepatic glycogen-targeting subunit G/L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.1 kDa (308aa)

Additionnal Information

PPP1R3B, Protein phosphatase 1 regulatory subunit 3B, GL, PPP1R4, PTG, ATGP2330-10 µg, ATGP2330-20 µg, ATGP2330-50 µg, ATGP2330-100 µg, ATGP2330-250 µg, ATGP2330-500 µg, ATGP2330-1 mg, ATGP2330-10, ATGP2330-20, ATGP2330-50, ATGP2330-100, ATGP2330-250, ATGP2330-500, ATGP2330-1

References & Citations

Munro S., et al. (2005) Diabetes. 51:591-598; ; Ota T., et al. (2004) Nat. Genet. 36:40-45;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001188258

Uniprot Accession Number

Q86XI6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMMAVDIE YRYNCMAPSL RQERFAFKIS PKPSKPLRPC IQLSSKNEAS GMVAPAVQEK KVKKRVSFAD NQGLALTMVK VFSEFDDPLD MPFNITELLD NIVSLTTAES ESFVLDFSQP SADYLDFRNR LQADHVCLEN CVLKDKAIAG TVKVQNLAFE KTVKIRMTFD TWKSYTDFPC QYVKDTYAGS DRDTFSFDIS LPEKIQSYER MEFAVYYECN GQTYWDSNRG KNYRIIRAEL KSTQGMTKPH SGPDLGISFD QFGSPRCSYG LFPEWPSYLG YEKLGPYY