Recombinant human SPRY4 protein

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS. Recombinant human SPRY4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Protein sprouty homolog 4 isoform 2, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila)

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.9 kDa (322aa)

Additionnal Information

SPRY4, Protein sprouty homolog 4 isoform 2, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila), ATGP2326-10 µg, ATGP2326-20 µg, ATGP2326-50 µg, ATGP2326-100 µg, ATGP2326-250 µg, ATGP2326-500 µg, ATGP2326-1 mg, ATGP2326-10, ATGP2326-20, ATGP2326-50, ATGP2326-100, ATGP2326-250, ATGP2326-500, ATGP2326-1

References & Citations

Katoh Y, Katoh M. (2006) . Int J Mol Med 17 (3) : 529-32

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001120968

Uniprot Accession Number

Q9C004

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEPPIPQ SAPLTPNSVM VQPLLDSRMS HSRLQHPLTI LPIDQVKTSH VENDYIDNPS LALTTGPKRT RGGAPELAPT PARCDQDVTH HWISFSGRPS SVSSSSSTSS DQRLLDHMAP PPVADQASPR AVRIQPKVVH CQPLDLKGPA VPPELDKHFL LCEACGKCKC KECASPRTLP SCWVCNQECL CSAQTLVNYG TCMCLVQGIF YHCTNEDDEG SCADHPCSCS RSNCCARWSF MGALSVVLPC LLCYLPATGC VKLAQRGYDR LRRPGCRCKH TNSVICKAAS GDAKTSRPDK PF