Recombinant human KAP1 protein

TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Recombinant human TRIM28 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Transcription intermediary factor 1-beta, Transcription intermediary factor 1-beta, Tripartite motif containing 28, KAP1, RNF96, TF1B, TIF1B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.7 kDa (460aa)

Additionnal Information

KAP1, Transcription intermediary factor 1-beta, Transcription intermediary factor 1-beta, Tripartite motif containing 28, KAP1, RNF96, TF1B, TIF1B, ATGP2325-10 µg, ATGP2325-20 µg, ATGP2325-50 µg, ATGP2325-100 µg, ATGP2325-250 µg, ATGP2325-500 µg, ATGP2325-1 mg, ATGP2325-10, ATGP2325-20, ATGP2325-50, ATGP2325-100, ATGP2325-250, ATGP2325-500, ATGP2325-1

References & Citations

Friedman J.R, . et al. (1996) . Genes Dev. 10:2067-2078

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005753

Uniprot Accession Number

Q13263

Species

Human

AA Sequence

KLIYFQL HRALKMIVDP VEPHGEMKFQ WDLNAWTKSA EAFGKIVAER PGTNSTGPAP MAPPRAPGPL SKQGSGSSQP MEVQEGYGFG SGDDPYSSAE PHVSGVKRSR SGEGEVSGLM RKVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVI ERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTS APGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQR KCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFF ETRMNEAFGD