Recombinant human AP1AR protein

Adaptor-related protein complex 1 associated regulatory protein, also known as AP1AR, is necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. This protein regulates the membrane association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with AP1G1/Gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Recombinant human AP1AR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Adaptor-related protein complex 1 associated regulatory protein, Adaptor-related protein complex 1 associated regulatory protein, 2C18, C4orf16, gamma-BAR, GBAR, PRO0971

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.4 kDa (322aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

AP1AR, Adaptor-related protein complex 1 associated regulatory protein, Adaptor-related protein complex 1 associated regulatory protein, 2C18, C4orf16, gamma-BAR, GBAR, PRO0971, ATGP2321-10 µg, ATGP2321-20 µg, ATGP2321-50 µg, ATGP2321-100 µg, ATGP2321-250 µg, ATGP2321-500 µg, ATGP2321-1 mg, ATGP2321-10, ATGP2321-20, ATGP2321-50, ATGP2321-100, ATGP2321-250, ATGP2321-500, ATGP2321-1

References & Citations

Neubrand V.E., et al. (2005) EMBO J. 24:1122-1133; ; Wollscheid B., et al. (2009) Nat. Biotechnol. 27:378-386;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_061039

Uniprot Accession Number

Q63HQ0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ TR