Recombinant human HS3ST1 protein
Heparan sulfate glucosamine 3-O-sulfotransferase 1, also known as HS3ST1, is sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. This protein catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact) . This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Recombinant human HS3ST1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate glucosamine 3-O-sulfotransferase 1, 3OST, 3OST1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.2 kDa (310aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Shworak N.W., et al. (1997) J. Biol. Chem. 272:28008-28019
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005105
Uniprot Accession Number
O14792
Species
Human
AA Sequence
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