Recombinant human NAP1L4 protein

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15. 5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Recombinant human NAP1L4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nucleosome assembly protein 1-like 4, hNAP2, NAP1L4b, NAP2, NAP2L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.2 kDa (398aa) confirmed by MALDI-TOF

Additionnal Information

NAP1L4, Nucleosome assembly protein 1-like 4, hNAP2, NAP1L4b, NAP2, NAP2L, ATGP2317-10 µg, ATGP2317-20 µg, ATGP2317-50 µg, ATGP2317-100 µg, ATGP2317-250 µg, ATGP2317-500 µg, ATGP2317-1 mg, ATGP2317-10, ATGP2317-20, ATGP2317-50, ATGP2317-100, ATGP2317-250, ATGP2317-500, ATGP2317-1

References & Citations

Olsen J.V., et al. (2006) Cell. 127:635-648; ; Nousiainen M., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:5391-5396;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005960

Uniprot Accession Number

Q99733

Species

Human

AA Sequence

MADHSFS DGVPSDSVEA AKNASNTEKL TDQVMQNPRV LAALQERLDN VPHTPSSYIE TLPKAVKRRI NALKQLQVRC AHIEAKFYEE VHDLERKYAA LYQPLFDKRR EFITGDVEPT DAESEWHSEN EEEEKLAGDM KSKVVVTEKA AATAEEPDPK GIPEFWFTIF RNVDMLSELV QEYDEPILKH LQDIKVKFSD PGQPMSFVLE FHFEPNDYFT NSVLTKTYKM KSEPDKADPF SFEGPEIVDC DGCTIDWKKG KNVTVKTIKK KQKHKGRGTV RTITKQVPNE SFFNFFNPLK ASGDGESLDE DSEFTLASDF EIGHFFRERI VPRAVLYFTG EAIEDDDNFE EGEEGEEEEL EGDEEGEDED DAEINPKV