Recombinant human NDUFV2 protein
The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. NDUFV2 is the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19. Recombinant human NDUFV2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
NADH dehydrogenase [ubiquinone] flavoprotein 2, CI-24k, NADH:ubiquinone oxidoreductase core subunit V2, Complex I 24kDa subunit
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.1 kDa (240aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Murray J., et al. (2003) J. Biol. Chem. 278:13619-13622; ; Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_066552
Uniprot Accession Number
P19404
Species
Human
AA Sequence
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