Recombinant human CD43 protein

SPN is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. Recombinant human SPN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Leukosialin precursor, CD43, GALGP, GPL115, LSN

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.8 kDa (257aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CD43, leukosialin precursor, CD43, GALGP, GPL115, LSN, ATGP2310-10 µg, ATGP2310-20 µg, ATGP2310-50 µg, ATGP2310-100 µg, ATGP2310-250 µg, ATGP2310-500 µg, ATGP2310-1 mg, ATGP2310-10, ATGP2310-20, ATGP2310-50, ATGP2310-100, ATGP2310-250, ATGP2310-500, ATGP2310-1

References & Citations

Park, H.J., et al. (2012) Am. J. Surg. Pathol. 36 (8), 1150-1157; ; Balikova, A., et al. (2012) Int. J. Oncol. 41 (1), 299-309

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_003114.1

Uniprot Accession Number

P16150

Species

Human

AA Sequence

STTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR