Recombinant human UPRT protein
UPRT is a uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (uMP) . This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. Recombinant human uPRT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Uracil phosphoribosyltransferase homolog, uracil phosphoribosyltransferase (FuR1) homolog (S.cerevisiae), FuR1, RP11-311P8.3, uPP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.2 kDa (332aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
T. Selwood and E. K. Jaffe. (2011) Arch. Biochem. Biophys. 519 (2) : 131-43.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_659489
Uniprot Accession Number
Q96BW1
Species
Human
AA Sequence
Frequently Asked Questions
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