Recombinant human UPRT protein

UPRT is a uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (uMP) . This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. Recombinant human uPRT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Uracil phosphoribosyltransferase homolog, uracil phosphoribosyltransferase (FuR1) homolog (S.cerevisiae), FuR1, RP11-311P8.3, uPP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.2 kDa (332aa) confirmed by MALDI-TOF

Additionnal Information

UPRT, uracil phosphoribosyltransferase homolog, uracil phosphoribosyltransferase (FuR1) homolog (S.cerevisiae), FuR1, RP11-311P8.3, uPP, ATGP2309-10 µg, ATGP2309-20 µg, ATGP2309-50 µg, ATGP2309-100 µg, ATGP2309-250 µg, ATGP2309-500 µg, ATGP2309-1 mg, ATGP2309-10, ATGP2309-20, ATGP2309-50, ATGP2309-100, ATGP2309-250, ATGP2309-500, ATGP2309-1

References & Citations

T. Selwood and E. K. Jaffe. (2011) Arch. Biochem. Biophys. 519 (2) : 131-43.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_659489

Uniprot Accession Number

Q96BW1

Species

Human

AA Sequence

MATELQC PDSMPCHNQQ VNSASTPSPE QLRPGDLILD HAGGNRASRA KVILLTGYAH SSLPAELDSG ACGGSSLNSE GNSGSGDSSS YDAPAGNSFL EDCELSRQIG AQLKLLPMND QIRELQTIIR DKTASRGDFM FSADRLIRLV VEEGLNQLPY KECMVTTPTG YKYEGVKFEK GNCGVSIMRS GEAMEQGLRD CCRSIRIGKI LIQSDEETQR AKVYYAKFPP DIYRRKVLLM YPILSTGNTV IEAVKVLIEH GVQPSVIILL SLFSTPHGAK SIIQEFPEIT ILTTEVHPVA PTHFGQKYFG TD