Recombinant human KIAA0101/PCLAF protein
KIAA0101 is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. This protein also acts as a regulator of centrosome number. Recombinant human KIAA0101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
PCNA clamp associated factor, PAF15, PAF, p15 (PAF), OEATC-1, OEATC, NS5ATP9, L5, KIAA0101
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
14.4 kDa (134aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kais Z., et al (2011) . Mol. Cancer Res. 9:1091-1099; ; Povlsen L.K., et al (2012) . Nat. Cell Biol. 14:1089-1098
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_055551.1
Uniprot Accession Number
Q15004
Species
Human
AA Sequence
MVRTKAD SVPGTYRKVV AARAPRKVLG SSTSATNSTS VSSRKAENKY AGGNPVCVRP TPKWQKGIGE FFRLSPKDSE KENQIPEEAG SSGLGKAKRK ACPLQPDHTN DEKE
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