Recombinant human CUEDC2 protein

CUEDC2 belongs to the CUEDC2 family and contains 1 CUE domain. This protein down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. It also involves in 17 beta-estradiol-induced ESR1 degradation and controls PGR protein levels through a similar mechanism. Recombinant human CUEDC2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CuE domain-containing protein 2, bA18I14.5, C10orf66

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.4 kDa (310aa) confirmed by MALDI-TOF

Additionnal Information

CUEDC2, CuE domain-containing protein 2, bA18I14.5, C10orf66, ATGP2299-10 µg, ATGP2299-20 µg, ATGP2299-50 µg, ATGP2299-100 µg, ATGP2299-250 µg, ATGP2299-500 µg, ATGP2299-1 mg, ATGP2299-10, ATGP2299-20, ATGP2299-50, ATGP2299-100, ATGP2299-250, ATGP2299-500, ATGP2299-1

References & Citations

Pan X, Zhou T, et al. (2011) . Nat Med. 17 (6) :708-14. ; ; Zhang PJ, Zhao J, et al. (2007) . EMBO J. 26 (7) :1831-42.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_076945

Uniprot Accession Number

Q9H467

Species

Human

AA Sequence

MELERIV SAALLAFVQT HLPEADLSGL DEVIFSYVLG VLEDLGPSGP SEENFDMEAF TEMMEAYVPG FAHIPRGTIG DMMQKLSGQL SDARNKENLQ PQSSGVQGQV PISPEPLQRP EMLKEETRSS AAAAADTQDE ATGAEEELLP GVDVLLEVFP TCSVEQAQWV LAKARGDLEE AVQMLVEGKE EGPAAWEGPN QDLPRRLRGP QKDELKSFIL QKYMMVDSAE DQKIHRPMAP KEAPKKLIRY IDNQVVSTKG ERFKDVRNPE AEEMKATYIN LKPARKYRFH