Recombinant human NEURL2 protein

NEuRL2 plays an important role in the process of myofiber differentiation and maturation. This protein is probable substrate-recognition component of a SCF-like ECS (Elongin BC-CuL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, which mediates the ubiquitination of proteins. It probably contributes to catalysis through recognition and positioning of the substrate and the ubiquitin-conjugating enzyme. During myogenesis, NEuRL2 controls the ubiquitination and degradation of the specific pool of CTNNB1/beta-catenin located at the sarcolemma. Recombinant human NEuRL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neuralized homolog 2, C20orf163, OZZ, Ozz-E3, Neuralized E3 ubiquitin protein ligase 2, Neuralized-like 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.1 kDa (308aa)

Additionnal Information

Neuralized homolog 2, C20orf163, OZZ, Ozz-E3, Neuralized E3 ubiquitin protein ligase 2, Neuralized-like 2, NEuRL2, Ozz E3, Neuralized like 2, ATGP2297-10 µg, ATGP2297-20 µg, ATGP2297-50 µg, ATGP2297-100 µg, ATGP2297-250 µg, ATGP2297-500 µg, ATGP2297-1 mg, ATGP2297-010, ATGP2297-020, ATGP2297-050, ATGP2297-100, ATGP2297-250, ATGP2297-500, ATGP2297-01M

References & Citations

Aurino, S., et al. (2008) Acta Myol 27, 90-97; ; Song, R., et al. (2006) J. Biol. Chem. 281 (47), 36391-36400

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_542787

Uniprot Accession Number

Q9BR09

Species

Human

AA Sequence

MAAASEP VDSGALWGLE RPEPPPTRFH RVHGANIRVD PSGTRATRVE SFAHGVCFSR EPLAPGQVFL VEIEEKELGW CGHLRLGLTA LDPASLAPVP EFSLPDLVNL GHTWVFAITR HHNRVPREGR PEAEAAAPSR PPTLLVEPYL RIEQFRIPRD RLVGRSRPGL YSHLLDQLYE LNVLPPTARR SRLGVLFCPR PDGTADMHII INGEDMGPSA RGLPAAQPLY AVVDVFASTK SVRLVQLEYG LPSLQTLCRL VIQRSMVHRL AIDGLHLPKE LKDFCKYE