Recombinant human Granzyme K protein

GZMK is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. Recombinant human GZMK protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Granzyme K, GZMK, TRYP2, GZMK, PRSS, tryptase II, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.2 kDa (261aa)

Additionnal Information

GZMK, Granzyme K, TRYP2, ATGP2294-10 µg, ATGP2294-20 µg, ATGP2294-50 µg, ATGP2294-100 µg, ATGP2294-250 µg, ATGP2294-500 µg, ATGP2294-1 mg, ATGP2294-10, ATGP2294-20, ATGP2294-50, ATGP2294-100, ATGP2294-250, ATGP2294-500, ATGP2294-1

References & Citations

Rucevic M, Fast LD, et al. (2007) . Shock. 27 (5) :488-93.; ; Zhao T, Zhang H, et al. (2007) . J Biol Chem. 282 (16) :12104-11.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002095.1

Uniprot Accession Number

P49863

Species

Human

AA Sequence

IIGGKEV SPHSRPFMAS IQYGGHHVCG GVLIDPQWVL TAAHCQYRFT KGQSPTVVLG AHSLSKNEAS KQTLEIKKFI PFSRVTSDPQ SNDIMLVKLQ TAAKLNKHVK MLHIRSKTSL RSGTKCKVTG WGATDPDSLR PSDTLREVTV TVLSRKLCNS QSYYNGDPFI TKDMVCAGDA KGQKDSCKGD SGGPLICKGV FHAIVSGGHE CGVATKPGIY TLLTKKYQTW IKSNLVPPHT N