Recombinant human RNF7 protein
RNF7 is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII) . The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHuK/IKK-alpha/IKBKA) and p27Kip1 (CDKN1B) . Alternatively spliced transcript variants encoding distinct isoforms have been reported. Recombinant human RNF7 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
RING-box protein 2 isoform 1, Ring finger protein 7, CKBBP1, ROC2, SAG
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.1 kDa (136aa)
Additionnal Information
References & Citations
Swaroop M., et al. (2000) . Oncogene 19:2855-2866
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Scientific Category
Metabolism
NCBI Accession Number
NP_055060
Uniprot Accession Number
Q9UBF6
Species
Human
AA Sequence
MADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK
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