Recombinant human PPM1F protein

PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

52.2 kDa (477aa)

Additionnal Information

PPM1F, Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2, ATGP2281-10 µg, ATGP2281-20 µg, ATGP2281-50 µg, ATGP2281-100 µg, ATGP2281-250 µg, ATGP2281-500 µg, ATGP2281-1 mg, ATGP2281-10, ATGP2281-20, ATGP2281-50, ATGP2281-100, ATGP2281-250, ATGP2281-500, ATGP2281-1

References & Citations

Tan KM, Chan SL, et al. (2001) . J Biol Chem. 276 (47) :44193-202. ; ; Harvey BP, Banga SS, et al. (2004) . J Biol Chem. 279 (23) :24889-98.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055449

Uniprot Accession Number

P49593

Species

Human

AA Sequence

MSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS