Recombinant human Osteoactivin/GPNMB protein
GPNMB is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. The protein shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GPNMB protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
53.2 kDa (476aa)
Additionnal Information
References & Citations
Rose A.A., et al. (2010) PLoS ONE. 5:e12093-e12093; ; Patel-Chamberlin M., et al. (2011) Kidney Int. 79:1138-1148
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_002501
Uniprot Accession Number
Q14956
Species
Human
AA Sequence
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