Recombinant human IGFBP-6 protein

IGFBP6 contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Recombinant human IGFPB6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Insulin-like growth factor-binding protein 6, IBP6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

25 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

IGFBP6, Insulin-like growth factor-binding protein 6, IBP6, ATGP2270-10 µg, ATGP2270-20 µg, ATGP2270-50 µg, ATGP2270-100 µg, ATGP2270-250 µg, ATGP2270-500 µg, ATGP2270-1 mg, ATGP2270-10, ATGP2270-20, ATGP2270-50, ATGP2270-100, ATGP2270-250, ATGP2270-500, ATGP2270-1

References & Citations

Shimasaki S., et al (1991), Mol. Endocrinol. 5:938-948; ; Andress D.L., et al (1991), Biochem. Biophys. Res. Commun. 176:213-218 (1991)

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_002169.1

Uniprot Accession Number

P24592

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSRCPGCGQ GVQAGCPGGC VEEEDGGSPA EGCAEAEGCL RREGQECGVY TPNCAPGLQC HPPKDDEAPL RALLLGRGRC LPARAPAVAE ENPKESKPQA GTARPQDVNR RDQQRNPGTS TTPSQPNSAG VQDTEMGPCR RHLDSVLQQL QTEVYRGAQT LYVPNCDHRG FYRKRQCRSS QGQRRGPCWC VDRMGKSLPG SPDGNGSSSC PTGSSG