Recombinant human IMPACT protein
IMPACT belongs to the IMPACT family and contains 1 RWD domain. This protein is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Recombinant human IMPACT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Protein IMPACT, RWDD5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.9 kDa (343aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Habibi, D., et al. (2010) J. Cell. Physiol. 225 (1), 196-205; ; Pereira, C.M., et al. (2005) J. Biol. Chem. 280 (31), 28316-28323
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_060909
Uniprot Accession Number
Q9P2X3
Species
Human
AA Sequence
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