Recombinant human RNA polymerase II subunit RPB4/POLR2D protein
POLR2D is the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. Recombinant human POLR2D protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, RBP4, RPB16
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
18.7 kDa (165aa)
Additionnal Information
References & Citations
Khazak V., et al. (1998) . Mol. Cell. Biol. 18:1935-1945
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004796
Uniprot Accession Number
O15514
Species
Human
AA Sequence
Frequently Asked Questions
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